fastp
seqtk
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fastp
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R pipelines for bulk RNA-seq analyses
fastp + multiQC + Salmon + DESeq2 all some nextflow workflow. It is a good exercise (not complicated) to create the pipeline from scratch the first time to properly understand each tool.
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NHI Genome Studies: Mexico Govt Sept 12 Congressional hearing
1) QC the data with fastp. This'll trim out adapters and toss reads that are poor quality.
- Illumina adapters and quality trimming
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Low-complexity sequence filtering tool
fastp has an adjustable low complexity filter option.
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Can you evaluate my pipeline?
- in terms of preprocessing and QC, I prefer fastp (https://github.com/OpenGene/fastp)
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Current QC tools for short read and long read sequencing
I generally use fastp as an all-in-one tool for short reads: https://github.com/OpenGene/fastp
- Qurstion about automating trimming process
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What methods (conda installable only please) can you use to determine the complexity of a fastq file? (e.g., kmer analysis)
I don't know if this fits exactly what you need, but I'm using fastp to check my fastq.gz files lately: https://github.com/OpenGene/fastp. You can install it via conda.
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A tool to count basepair in fastq file
If you also need some other basic statistics or want to filter the reads you can try fastp (https://github.com/OpenGene/fastp). If only the basepair count is needed, awk might be the fastest solution as suggested before.
seqtk
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Illumina adapters and quality trimming
seqtk: A lightweight and versatile tool for processing FASTQ and FASTA files. https://github.com/lh3/seqtk
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looking for a tool to filter non-coding regions/excise ORFs from a draft assembly
Perhaps seqtk could be helpful https://github.com/lh3/seqtk
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Help with understanding awk code
You could also check out tools specialized for FASTA processing like https://github.com/shenwei356/seqkit and https://github.com/lh3/seqtk
- !help
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Doubts with my first ever mRNA-seq QC analysis
If I were to analyze I would use a random fastq sampler like Seqtk and bring all your samples to a lowest read depth of your 27 libraries although I wouldn't analyze a library with less than 2mil reads. 5 mil is fine for differential, you can obviously get more reads and probably received more information but increasing read depth may plateau.
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Reverse sequencing of fastq file
It's a little toolkit written by one of the Illuminati of the Bioinformatics world: seqtk on GH
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[Help] Copying head of fastq file into a .txt file named .fastq, doesn't include the header resolving in an error when converting to .bam file.
I recommend installing seqtk, which makes this easy. Of course sed/awk/perl are theoretically entirely sufficient but why make life more difficult than necessary?
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Looking for small SRA Data Sets
Most SRA files are grouped by projects. On a basic level for something common like RNA-seq you will have replicates of the control and treatment/diseased samples. Each file (i.e. sample) contains raw sequencing reads, usually millions per sample. You could randomly subsample the sequencing reads very easily using many tools (common choice is https://github.com/lh3/seqtk). There is no way you are going to assemble an animal genome with MB file sizes (for example the human genome itself is already over 3GB in size). You should probably look for bacterial or viral DNA samples and subset those to an appropriate size.
What are some alternatives?
galaxy - Data intensive science for everyone.
seqkit - A cross-platform and ultrafast toolkit for FASTA/Q file manipulation
readfq - A simple tool to calculate reads number and total base count in FASTQ file
samtools - [Moved to: https://github.com/ingolia/SamTools]
glslSmartDeNoise - Fast glsl deNoise spatial filter, with circular gaussian kernel, full configurable
htslib - C library for high-throughput sequencing data formats
nextclade - Viral genome alignment, mutation calling, clade assignment, quality checks and phylogenetic placement
samtools - Tools (written in C using htslib) for manipulating next-generation sequencing data
readfq - Fast multi-line FASTA/Q reader in several programming languages
minimap2 - A versatile pairwise aligner for genomic and spliced nucleotide sequences
fasql - DuckDB Extension for reading and writing FASTA and FASTQ Files
bam-filter - Use simple expressions to filter a BAM/CRAM file