minimap2 VS surge

Interested as well! But the future is not so dark, things like e.g. https://github.com/lh3/minimap2 are a breath of fresh air.

No experience with this maybe try minimap2(https://github.com/lh3/minimap2) if this doesn't work fall back on blast/blat

You can try minimap2 to align your long reads to your expected plasmid

It sounds like the mapping wasn't very good, you might want to try minimap2 as it is a newer algorithm.

Good stuff!

I started getting in to this at the start of the year. Already had an old, dusty MicroKORG and MIDI interface to use it as a controller, but recently splashed out on a bigger controller as the Korg's tiny keys were hurting me - plus, I wanted something bigger to get better at piano!

A couple of free soft synths I'd recommend are Surge XT, and Vital.

https://surge-synthesizer.github.io/

https://vital.audio/

https://surge-synthesizer.github.io/ and https://asb2m10.github.io/dexed/ should work like a charm.

To get the equivalent of a symphonic orchestra in your computer, the solution is basically money; you buy the instruments you need. In the case of synthesizers, things are much cheaper - if you put in the effort yourself. https://surge-synthesizer.github.io/ is excellent and could even be used if you wanted to make a more retro-style soundtrack.

Instead of Synth1, try https://surge-synthesizer.github.io/ . It's pretty much better in every aspect except for the UI which is going to look a lot more daunting to you ;)

In general, this should only happen when a Program Change message is sent.