minimap2
slivar
minimap2 | slivar | |
---|---|---|
5 | 1 | |
1,688 | 240 | |
- | - | |
7.6 | 5.7 | |
7 days ago | 5 months ago | |
C | Nim | |
GNU General Public License v3.0 or later | MIT License |
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minimap2
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Ask HN: Comment here about whatever you're passionate about at the moment
Interested as well! But the future is not so dark, things like e.g. https://github.com/lh3/minimap2 are a breath of fresh air.
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BLAST 10,000 genes?
No experience with this maybe try minimap2(https://github.com/lh3/minimap2) if this doesn't work fall back on blast/blat
- Truncating genome fastas to just overlapping regions
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Alignment of long reads to plasmid and generation of consensus sequence.
You can try minimap2 to align your long reads to your expected plasmid
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Questions about WGS mapping
It sounds like the mapping wasn't very good, you might want to try minimap2 as it is a newer algorithm.
slivar
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Help on installation of GEMINi
Looks like they’ve broken the install at some point. If you check the GitHub for Gemini it recommends you replace it with slivar https://github.com/brentp/slivar
What are some alternatives?
bwa-mem2 - The next version of bwa-mem
deepvariant - DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data.
seqtk - Toolkit for processing sequences in FASTA/Q formats
bwa - Burrow-Wheeler Aligner for short-read alignment (see minimap2 for long-read alignment)
seqstats - Quick summary statistics on fasta/fastq(.gz) files
rare-disease-wf - (WIP) best-practices workflow for rare disease
MethylDackel - A (mostly) universal methylation extractor for BS-seq experiments.
open-cravat - A modular annotation tool for genomic variants
bwa-mem2 - The next version of bwa-mem
Biopython - Official git repository for Biopython (originally converted from CVS)
Klib - A standalone and lightweight C library
codeql - GitHub Satellite 2020 workshops on finding security vulnerabilities with CodeQL for Java/JavaScript.