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Shameless self promotion, but my company released an open source library that reads fasta and fastq files in python or other languages... https://github.com/wheretrue/fasql -- obv biased, but it's faster than biopython and has a lower footprint when you just need that.
Heng Li has a FASTQ/FASTA reader that I generally cut and paste into my code rather than use Biopython. Biopython has a very rich model for sequence data but you generally don't need 90% of it and it comes at a significant performance cost.
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Hence, a higher number means a more popular project.