hgvs
mixcr
hgvs | mixcr | |
---|---|---|
1 | 1 | |
231 | 310 | |
1.3% | 2.6% | |
8.3 | 9.7 | |
7 days ago | 3 days ago | |
Python | Kotlin | |
Apache License 2.0 | GNU General Public License v3.0 or later |
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hgvs
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[Question] How to transform HGVSg to HGVSp?
Python > https://github.com/biocommons/hgvs
mixcr
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Is it possible to get tcra data from single cell raw reads? The paper only provides tcrb data.
If you have access to the fqs then there's a chance you can extract some sequences out using Mixcr or similar, but there probably won't be many. You will need the reads because not all of the TCR is germline encoded.
What are some alternatives?
16SMaRT - 16s rRNA Sequencing Meta-analysis Reproducibility Tool (using mothur).
salmon - 🐟 🍣 🍱 Highly-accurate & wicked fast transcript-level quantification from RNA-seq reads using selective alignment
ariba - Antimicrobial Resistance Identification By Assembly
galaxy - Data intensive science for everyone.
rnaseq - RNA-seq analyses.
fastp - An ultra-fast all-in-one FASTQ preprocessor (QC/adapters/trimming/filtering/splitting/merging...)
alevin-fry - 🐟 🔬🦀 alevin-fry is an efficient and flexible tool for processing single-cell sequencing data, currently focused on single-cell transcriptomics and feature barcoding.
deepvariant - DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data.