Biopython VS weblogo

Usearch will do all-against-all comparisons, cluster sequences, and produce alignments for each cluster. You can set the clustering threshold (proportion of residues identical). The alignments are in fasta format, which is pretty standard. If all you want is basic similarity it might be easiest to just write something that calculates normalized Hamming distances (typically called p-distances in the molecular evolution literature) between pairs of sequences. I suspect the biopython fasta reader (you can install biopython from https://biopython.org/) will be good enough.

Yes, the computing tool for biological computation.

Beside that their core lectures to non-computer scientists are public (survey), workshops by software carpentry move around the globe. Maybe your intent to seed hands-on knowledge is in similar tune before heading for biopython, bioperl, bioawk. It doesn't hurt to tap into resources initially written for non-labrats either, e.g. about regular expressions by programming historian.

Well, what language do you want to learn? What is your background so far? Assuming it is more on the side of biology, software carpentry's Python may eventually lead to biopython? Though there equally is a chance for AWK (Hack the planet's text! and bioawk...

I think you mind find something of a community around BioPython, which might be helpful. Just looking at the capabilities will probably be instructive as well.

Python is the main programming language I use nowadays. In particular numpy and pandas are of course extremely useful. I also use biopython package - a collection of software tools for biological computation written in Python by an international group of researchers and developers.

To get the consensus of splice motifs I usually don't do motif discovery, because I already know where the motif is supposed to be. Instead, I get a window around the splice sites, extract the sequences and collect them in a fasta file. Then I upload the sequences in weblogo to visualize the sequence logo.

you can use this on the comnand line to make nice sequence logos: https://github.com/WebLogo/weblogo