drep | masurca | |
---|---|---|
1 | 1 | |
237 | 229 | |
- | - | |
3.9 | 3.5 | |
about 2 months ago | 2 months ago | |
Python | M4 | |
- | GNU General Public License v3.0 only |
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drep
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How to dereplicate MAGs? (metagenome assembly genomes)
You should look at https://github.com/MrOlm/drep . Thats exactly the tool you need. Usually one takes all their bins and dereplicates them at 99% ANI for strain level dereplication or 95% at species level. For preserving the best quality, you would need to check yourself if the best genome survived the dereplication afaik. You probably want some sort of quality score and chose the one with the highest score out of the bins that got grouped as one by dereplication.
masurca
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Nanopore long read assembly help!
Hybrid assembly: https://github.com/alekseyzimin/masurca
What are some alternatives?
DAS_Tool - DAS Tool
deepvariant - DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data.
SqueezeMeta - A complete pipeline for metagenomic analysis
bioinformatics - Bioinformatic algorithms for the UCLA Bioinformatics Specialization
covid-19-signal - Files and methodology pertaining to the sequencing and analysis of SARS-CoV-2, causative agent of COVID-19.
mag - Assembly and binning of metagenomes
kraken-biom - Create BIOM-format tables (http://biom-format.org) from Kraken output (http://ccb.jhu.edu/software/kraken/, https://github.com/DerrickWood/kraken).
Trycycler - A tool for generating consensus long-read assemblies for bacterial genomes
phantasm - PHANTASM: PHylogenomic ANalyses for the TAxonomy and Systematics of Microbes
aviary - A hybrid assembly and MAG recovery pipeline (and more!)
pyrodigal - Cython bindings and Python interface to Prodigal, an ORF finder for genomes and metagenomes. Now with SIMD!
eager - A fully reproducible and state-of-the-art ancient DNA analysis pipeline