drep
aviary
drep | aviary | |
---|---|---|
1 | 3 | |
237 | 67 | |
- | - | |
3.9 | 9.0 | |
about 2 months ago | 3 days ago | |
Python | Python | |
- | GNU General Public License v3.0 only |
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drep
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How to dereplicate MAGs? (metagenome assembly genomes)
You should look at https://github.com/MrOlm/drep . Thats exactly the tool you need. Usually one takes all their bins and dereplicates them at 99% ANI for strain level dereplication or 95% at species level. For preserving the best quality, you would need to check yourself if the best genome survived the dereplication afaik. You probably want some sort of quality score and chose the one with the highest score out of the bins that got grouped as one by dereplication.
aviary
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what would you choose to detect specific genes in metagenomics data
Aviary is a good binning pipeline (there are others available), though perhaps more geared towards prokaryotes.
- Why bother reconstructing MAGs ?
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question about analysis of metagenome assembled genomes
Have a look at this especially the workflow figure https://github.com/rhysnewell/aviary
What are some alternatives?
DAS_Tool - DAS Tool
metaWRAP - MetaWRAP - a flexible pipeline for genome-resolved metagenomic data analysis
SqueezeMeta - A complete pipeline for metagenomic analysis
metaGEM - :gem: An easy-to-use workflow for generating context specific genome-scale metabolic models and predicting metabolic interactions within microbial communities directly from metagenomic data
covid-19-signal - Files and methodology pertaining to the sequencing and analysis of SARS-CoV-2, causative agent of COVID-19.
masurca
kraken-biom - Create BIOM-format tables (http://biom-format.org) from Kraken output (http://ccb.jhu.edu/software/kraken/, https://github.com/DerrickWood/kraken).
sv-callers - Snakemake-based workflow for detecting structural variants in genomic data
phantasm - PHANTASM: PHylogenomic ANalyses for the TAxonomy and Systematics of Microbes