Identifying potential genes involved in nociception

• Liftoff

  2 392 5.2 Python

  An accurate GFF3/GTF lift over pipeline (by agshumate)

How distant is your study organism to Drosophila? You mention it's another model organism but I can't think of any others that are close to flies. If you have a close-ish organism you could try a 'lift' of the annotations from Drosophila to your draft genome (I like 'liftoff' for this). This tries to match across genes from your input reference (Drosophila) to your draft genome. This works best when the 2 organisms are somewhat closely related. You can then look up where those 'painless' genes in your draft organism's reference, and this info can also be used to inform your transcriptome analysis.

• miniprot

  1 304 5.8 C

  Align proteins to genomes with splicing and frameshift

Alternatively, there is a new tool out from Heng Li (bioinformatics godfather) which apparently does good quality mapping of proteins to references (miniprot). You could download your 'painless' genes from Drosophila and use this tool to look for them in your reference (similar to above, except this tool is apparently better able to handle larger differences).

• InfluxDB

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