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https://github.com/rasteri/dISAppointment
older similar project http://rayer.g6.cz/hardware/lpc_sio.htm
LPC is not without perils tho, there is trouble with DMA support as mentioned here https://flaterco.com/kb/ISA_chipsets.html
More about LPC bus https://blogs.coreboot.org/blog/2013/06/14/a-biology-laborat...
https://github.com/osnr/rpi-bitbang-ethernet
Might be possible, someone bitbanged (sending) ethernet frames.
This example makes me convinved that if you added a clock and some latches to a raspberry pi, it definitely should be able to do ISA bus.
Maybe even without any hardware, who knows. But it needs to level shift from 3V to 5V anyway.
I had some very successful experiments with bit banging ISA on a Raspberry Pi 3 and 4 a while ago: https://github.com/polpo/pigus I quickly moved over to a Raspberry Pi Pico because it's much better suited to such things. You need more than a couple I/O pins, though... to do real ISA (i.e., not LPC) right, you need more GPIOs than what the Pi gives you. But muxing the data/address lines is enough to give you back what you need.
Related posts
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PicoGUS: Emulate ISA Sound Cards (GUS, Adlib, MPU-401, Tandy, CMS) with a Pico
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Building a Raspberry Pi based ultrasound imaging development platform
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The (Almost) Definitive PC Sound Card Article (Pre-1990)
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PicoGUS January 2023 Firmware 0.3.0 update – it's DOOMed