gatk4-genome-processing-pipeline-azure VS trinityrnaseq

The GATK suite is pretty standard for calling germline mutations. Somatic mutation calling is a lot newer/trickier, so I'm just going to link to the GDC's practices.

This pre-processing pipeline from Microsoft (adapted from the Broad Institute/GATK) is pretty well-documented - at least in my opinion - with input requirements, expected outputs, software requirements, etc.

The screen shot suggests you are not able to actually compile/install trinity. The cmake command is not used to run it. After you download and unpack the trinity software package: https://github.com/trinityrnaseq/trinityrnaseq/releases/download/Trinity-v2.13.2/trinityrnaseq-v2.13.2.FULL.tar.gz, you should look at the INSTALL file, which tells you to:

I'm trying commands from the Docker site and the trinity site. When I try to run the command, it behaves like the code is incomplete (hitting enter just gives me a new line). To my understanding, the issue can be either in the pathway/to/files part of the code, of in the trinityrnaseq/trinityrnaseq part.

For my analysis I am trying to do variant calling on my transcriptome. I am following the Trinity github pipeline for variant calling, in which they provided a python script for GATK for the variant calling. I use SLURM to run this job for me:

Since you're an R user, I would recommend Bioconductor. Here's an excellent tutorial on how to analyze RNA-seq data (make sure that you're R version is up to date!): https://www.bioconductor.org/packages/devel/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html . However, if you want to assemble raw transcriptome data, then Trinity is a great tool (https://github.com/trinityrnaseq/trinityrnaseq/wiki).