Trimmomatic reviews and mentions
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Trimmomatic parameters
Yes it can produce results without the adapter sequences. But if you want to remove adapter sequences from your reads: 1) Find out which library prep kit and adapter sequences were used to create the library. 2) If it is common adapter sequence like TrueSeq you can find the adapter sequence file TrueSeq-PE.fa in the trimmomatic github page. (https://github.com/usadellab/Trimmomatic/tree/main/adapters). Or you can google it, illumina has a website that you can find all adapters. Then you need to download adapter sequences to your working directory. Also I suggest reading the documentation of trimmomatic for other paramaters. For adapter removal, trimmomatic paper contains detailed explaination for the algorithm.
- Experience in small/microRNA RNAseq? How to recognize adapter sequence?
Stats
Basic Trimmomatic repo stats
2
185
4.8
4 months ago
usadellab/Trimmomatic is an open source project licensed under GNU General Public License v3.0 or later which is an OSI approved license.
The primary programming language of Trimmomatic is Java.
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