Is it possible to assemble a complete bacterial genome using short reads?

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  • spades

    SPAdes Genome Assembler

  • MetaSpades has a cool option to hybrid reform contigs using short and long reads so you could pair short read data and long read data (PacBio/ONT) to get the best hybrid assembly with high throughput and long reference reads for resolving the reassembling. https://github.com/ablab/spades

  • mag

    Assembly and binning of metagenomes

  • InfluxDB

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