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pydna
Clone with Python! Data structures for double stranded DNA & simulation of homologous recombination, Gibson assembly, cut & paste cloning.
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pydna: https://github.com/BjornFJohansson/pydna
Julia (https://julialang.org/) might be worth trying if you care about execution speed (When doing big computations). But python is the better choice as a first language
NOTE:
The number of mentions on this list indicates mentions on common posts plus user suggested alternatives.
Hence, a higher number means a more popular project.