bwa-mem2
bowtie2
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bwa-mem2 | bowtie2 | |
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2 | 2 | |
676 | 618 | |
1.8% | - | |
2.6 | 7.6 | |
4 months ago | 4 days ago | |
C++ | C++ | |
GNU General Public License v3.0 or later | GNU General Public License v3.0 only |
Stars - the number of stars that a project has on GitHub. Growth - month over month growth in stars.
Activity is a relative number indicating how actively a project is being developed. Recent commits have higher weight than older ones.
For example, an activity of 9.0 indicates that a project is amongst the top 10% of the most actively developed projects that we are tracking.
bwa-mem2
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Anyone use DRAGEN-GATK?
If you haven’t heard of it already you may want to check out https://github.com/bwa-mem2/bwa-mem2 which is a faster version of bwa-mem. I’ve been using it for a while now and found it to be quite stable, same results as the original and the speed improvement is nice.
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Software Development Project
I’ve recently switched to bwa mem2 and the speed increase is nice for what is basically a drop in replacement (after a bit of validation to make sure that was true). https://github.com/bwa-mem2/bwa-mem2
bowtie2
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NHI Genome Studies: Mexico Govt Sept 12 Congressional hearing
2) Use bowtie2 to align reads against CHM13. This will let you separate human from nonhuman (important, as human sequences are a common contaminant in many nonhuman genomes).
- Computationally intensive steps in RNA-seq analysis
What are some alternatives?
minimap2 - A versatile pairwise aligner for genomic and spliced nucleotide sequences
STAR - RNA-seq aligner
minimap2 - A versatile pairwise aligner for genomic and spliced nucleotide sequences
megahit - Ultra-fast and memory-efficient (meta-)genome assembler
edlib - Lightweight, super fast C/C++ (& Python) library for sequence alignment using edit (Levenshtein) distance.
seq - A high-performance, Pythonic language for bioinformatics
htslib - C library for high-throughput sequencing data formats
fastp - An ultra-fast all-in-one FASTQ preprocessor (QC/adapters/trimming/filtering/splitting/merging...)
vg - tools for working with genome variation graphs
IntaRNA - Efficient target prediction incorporating accessibility of interaction sites
kraken2 - The second version of the Kraken taxonomic sequence classification system